Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YU-CHUN LIN
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 06:02:52
kit-3 facdex3+GFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2073007_sequence (Version 1) |
Description
this part contain BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_E0040(GFP)+BBa_B0012(ter) We transform this biobrick into E.coli strain BL21, and use IPTG promoter to control the fac-dex1 protein express. When this engineered bacteria be induced by IPTG overnight (at least 12 hours), it would show GFP fluorescence. Then we know the fac-dex1 is expressed by bacteria.Notes
If bacteria show GFP fluorescence, we could know the fac-dex3 is successfully expressedSource
BBa_J04500+BBa_K2073001+BBa_E0040+BBa_B0012| Access | Instance | Definition |
| public public public public | BBa_J04500 BBa_K2073001 BBa_E0040 BBa_B0012 | BBa_J04500 BBa_K2073001 GFP BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K2073001 BBa_E0040 BBa_B0012 | 1,220 227,1297 1304,2023 2032,2072 | BBa_J04500 BBa_K2073001 GFP BBa_B0012 |