BBa_K2080009

BBa_K2080009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2080009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Haojian Li

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 05:33:32

bax-sgRNA1234

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K2080009_sequence (Version 1)

Description

This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.

Notes

We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.

Source

From BBa_K2080001
BBa_K2080002
BBa_K2080003
BBa_K2080008

Components

Access	Instance	Definition
public	BBa_K2080001	BBa_K2080001
public	BBa_K2080002	BBa_K2080002
public	BBa_K2080003	BBa_K2080003
public	BBa_K2080008	BBa_K2080008

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2080001	1,367	BBa_K2080001
BBa_K2080002	376,742	BBa_K2080002
BBa_K2080003	751,1116	BBa_K2080003
BBa_K2080008	1125,1491	BBa_K2080008

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2080009/1

Member of these Collections

• iGEM Parts Registry