Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 05:51:21
bax-sgRNA-12
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K2080011_sequence (Version 1) |
Description
This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.Notes
We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.Source
FromBBa_K2080004
BBa_K2080005
| Access | Instance | Definition |
| public public | BBa_K2080004 BBa_K2080005 | BBa_K2080004 BBa_K2080005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2080004 BBa_K2080005 | 1,367 376,742 | BBa_K2080004 BBa_K2080005 |