BBa_K2080011

BBa_K2080011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2080011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Haojian Li

Date created: 2016-10-12 11:00:00

Date modified: 2016-10-13 05:51:21

bax-sgRNA-12

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K2080011_sequence (Version 1)

Description

This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-VP64 scaffold sequence, which can guide dCas9-VP64 to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bax, a proapoptosis protein, which can activate the transcription of bax.

Notes

We design the sequence of the gRNA that is complementary to the bax promoter region, and try to get closer to the transcription start site to obtain higher activation of bax trascription.

Source

From
BBa_K2080004
BBa_K2080005

Components

Access	Instance	Definition
public	BBa_K2080004	BBa_K2080004
public	BBa_K2080005	BBa_K2080005

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2080004	1,367	BBa_K2080004
BBa_K2080005	376,742	BBa_K2080005

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2080011/1

Member of these Collections

• iGEM Parts Registry