Types | DnaRegion
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Roles | engineered_region
Device
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Sequences | BBa_K2080013_sequence (Version 1)
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Description
This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.
Notes
We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bcl-2 trascription.
Source
From BBa_K2080004
BBa_K2080005