BBa_K2080013

BBa_K2080013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2080013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 05:56:37

bcl2-sgRNA-12



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K2080013_sequence (Version 1)

Description

This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.

Notes

We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bcl-2 trascription.

Source

From BBa_K2080004
BBa_K2080005

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2080013/1