Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Haojian Li
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 05:56:37
bcl2-sgRNA-12
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K2080013_sequence (Version 1) |
Description
This is a group of guide RNAs that have a hundred base-long molecule with a dCas9-KRAB scaffold sequence, which can guide dCas9-KRAB to its complementary part of a double-strand DNA. In our project, this gRNA is targeting to the promoter of bcl-2, a anti-apoptosis protein, which can inhibit the transcription of bcl-2.Notes
We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bcl-2 trascription.Source
From BBa_K2080004BBa_K2080005
| Access | Instance | Definition |
| public public | BBa_K2080004 BBa_K2080005 | BBa_K2080004 BBa_K2080005 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2080004 BBa_K2080005 | 1,367 376,742 | BBa_K2080004 BBa_K2080005 |