Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Le He
Date created: 2016-10-05 11:00:00
Date modified: 2016-10-12 09:13:08
inclusion body induced cell lysis system
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K2110008_sequence (Version 1) |
Description
Long Description: This part consists of theCpxR responsive promoter (Part:BBa_K135000), a RBS (Part:BBa_B0034), ddpX gene (Part: BBa_K2110004), two terminators (Part:BBa_B0010, Part:BBa_B0012). The CpxR responsive promoter can response to the inclusion body and misfold protein in the periplasm. When this system coexpress with an exogenous gene like the PETase gene, the forming inclusion body will induce the expression of downstream ddpX gene and cause the cell lysis, which provides much convenience to the purification steps followed.Notes
/Source
BBa_K135000BBa_B0034
BBa_K2110004
| Access | Instance | Definition |
| public public public | BBa_K135000 BBa_B0034 BBa_K2110004 | BBa_K135000 BBa_B0034 BBa_K2110004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K135000 BBa_B0034 BBa_K2110004 | 1,55 64,75 84,665 | BBa_K135000 BBa_B0034 BBa_K2110004 |