BBa_K2110008

BBa_K2110008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2110008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Le He

Date created: 2016-10-05 11:00:00

Date modified: 2016-10-12 09:13:08

inclusion body induced cell lysis system

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K2110008_sequence (Version 1)

Description

Long Description: This part consists of theCpxR responsive promoter (Part:BBa_K135000), a RBS (Part:BBa_B0034), ddpX gene (Part: BBa_K2110004), two terminators (Part:BBa_B0010, Part:BBa_B0012). The CpxR responsive promoter can response to the inclusion body and misfold protein in the periplasm. When this system coexpress with an exogenous gene like the PETase gene, the forming inclusion body will induce the expression of downstream ddpX gene and cause the cell lysis, which provides much convenience to the purification steps followed.

Notes

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Source

BBa_K135000
BBa_B0034
BBa_K2110004

Components

Access	Instance	Definition
public	BBa_K135000	BBa_K135000
public	BBa_B0034	BBa_B0034
public	BBa_K2110004	BBa_K2110004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K135000	1,55	BBa_K135000
BBa_B0034	64,75	BBa_B0034
BBa_K2110004	84,665	BBa_K2110004

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2110008/1

Member of these Collections

• //function/celldeath
• iGEM Parts Registry