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BBa_K2115001

BBa_K2115001 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K2115001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Antonella Elizabeth Osses Toledo

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 02:13:15

Third level of detection of carbon monoxide

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K2115001_sequence (Version 1)

Description

This third level of detection functions due to a quorum sensing system, when the lux genes for low and high levels are activated a pLux promoter wiil transcribe a green fluorescence protein.

Notes

Coordinating the action of two promoters in order to activate pLux.

Source

Plux: a repressor system that come from E.coli bacterium.
RBS: ribosome binding site
Greeb fluorescenc protein: we took GFP from the IGEM catalog.
Terminators:an RNA sequence that usually occurs at the end of a gene or operon mRNA and causes transcription to stop

Components

Access	Instance	Definition
public public public public public	BBa_B0030 BBa_C0061 BBa_E0040 BBa_B0010 BBa_B0012	BBa_B0030 luxI GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0030 BBa_C0061 BBa_E0040 BBa_B0010 BBa_B0012	1,15 22,639 671,1390 1399,1478 1487,1527	BBa_B0030 luxI GFP BBa_B0010 BBa_B0012