Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oskar ??hman
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 10:13:53
Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2144012_sequence (Version 1) |
Description
Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation.The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. The Linker in the LT-sequence enables proper folding of the protein.
Notes
Overhang PCR was performed to insert the Linker-tag-sequence.Source
-| Access | Instance | Definition |
| public public | BBa_K2144010 BBa_K2144009 | BBa_K2144010 BBa_K2144009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2144010 BBa_K2144009 | 1,873 882,932 | BBa_K2144010 BBa_K2144009 |