BBa_K2144012

BBa_K2144012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2144012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oskar ??hman
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 10:13:53

Coding sequence for ESP with His6 and LPXTG-tag regulated by T7-promoter



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K2144012_sequence (Version 1)

Description

Esp, extracellular serine protease, is capable of inhibit the biofilm formation and nasal colonization of S. aureus. This by protelysing receptors essential for biofilm formation and prevention of eDNA exudation.
The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed. The Linker in the LT-sequence enables proper folding of the protein.

Notes

Overhang PCR was performed to insert the Linker-tag-sequence.

Source

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igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2144012/1