Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oskar ??hman
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 03:02:53
Coding sequence for Nuclease with His6 and LPXTG-tag regulated by T7-promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K2144100_sequence (Version 1) |
Description
Coding sequence for Nuclease from Staphylococcus Aureus, regulated by T7-promoter. This enzyme has the ability to cleave DNA.The inserted LT-sequence contain the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Furthermore it contains the LXPTG-tag which enables the use of the "match making" enzyme Sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. A new peptide bond is formed.
The Linker in the LT-sequence enables proper folding of the protein.
Notes
The LT-sequence was inserted with Overhang PCR.Source
Nuclease is found in S.Aureus. The LT-sequence is ordered from IDT.| Access | Instance | Definition |
| public public | BBa_K2144000 BBa_K2144009 | Nuc-T7 BBa_K2144009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2144000 BBa_K2144009 | 1,599 608,658 | Nuc-T7 BBa_K2144009 |