BBa_K2150026

BBa_K2150026 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K2150026

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jianyi Huang

Date created: 2016-10-11 11:00:00

Date modified: 2016-10-17 08:48:53

pT7+pTet+RBS+tetX-GFP(fusion protein)+RBS+T7RNAP+DT

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K2150026_sequence (Version 1)

Description

A positive feedback device to over-express the fusion protein of tetX and GFP.

Notes

PT7 is placed upstream of pTet, regarding that pT7 is a strong promoter while pTet is relatively weak.
Linker for fusion protein is carefully selected to avoid repeated sequence that may cause problems in assembly.

Source

pT7:PCR amplified from BBa_K525998;T7RNAP:PCR amplified from genome of BL21.

As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).

pTet from BBa_R0040, DT from BBa_B0015,RBS from BBa_B0034

Components

Access	Instance	Definition
public	BBa_K2150031	pT7
public	BBa_R0040	p(tetR)
public	BBa_B0034	BBa_B0034
public	BBa_K2150013	tetX-GFP
public	BBa_B0034	BBa_B0034
public	BBa_I2032	BBa_I2032
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K2150031	1,20	pT7
BBa_R0040	29,82	p(tetR)
BBa_B0034	91,102	BBa_B0034
BBa_K2150013	109,2043	tetX-GFP
BBa_B0034	2052,2063	BBa_B0034
BBa_I2032	2070,4724	BBa_I2032
BBa_B0015	4733,4861	BBa_B0015

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2150026/1

Member of these Collections

• iGEM Parts Registry