Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jianyi Huang
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-17 08:49:56
pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K2150029_sequence (Version 1) |
Description
This part is a complete regulatory device sensitive to tetracycline.Notes
Two different terminators are used to avoid repeated sequence.Linker for fusion protein is carefully designed to avoid repeated sequence which may cause problems in assembly.
Source
pT7:PCR amplified from BBa_K525998;T7RNAP:PCR amplified from genome of BL21.As for fusion protein tetX-GFP, tetX is the same as (xxx), the protein linker is provided by our advisor Li Hua, and the GFP is from BBa_E0040. We combine this three parts using overlap extension polymerase chain reaction (OE-PCR).
| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_J23119 BBa_B0034 BBa_C0040 BBa_B0015 BBa_R0040 BBa_B0034 BBa_I2032 BBa_B0013 BBa_K2150031 BBa_B0034 BBa_K2150013 BBa_B0015 | BBa_J23119 BBa_B0034 tetR BBa_B0015 p(tetR) BBa_B0034 BBa_I2032 BBa_B0013 pT7 BBa_B0034 tetX-GFP BBa_B0015 |