Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ella Coleman
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 04:44:45
Chlorogenate Esterase gene
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2187001_sequence (Version 1) |
Description
The chlorogenate esterase gene comes from the Ideonella sakaiensis bacteria. Its purpose is to create an enzyme called PETase which breaks down PET into MHET.Notes
We added a lacl regulated promoter, BBa_R0011, to allow us to turn up enzyme production. In addition, we added a prefix and suffix, a BBa_B0030 RBS, and a BBa_K864600. We also had to change two internal Pst1 sites.Source
We used the genomic sequence of DNA: GAP38911.1 from GenBank.| Access | Instance | Definition |
| public public public public | BBa_R0011 BBa_K2187003 BBa_B0030 BBa_K864600 | lacI+pL BBa_K2187003 BBa_B0030 BBa_K864600 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_K2187003 BBa_B0030 BBa_K864600 | 1,54 64,2040 2049,2063 2072,2123 | lacI+pL BBa_K2187003 BBa_B0030 BBa_K864600 |