Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Shen
Date created: 2009-09-18 11:00:00
Date modified: 2015-05-08 01:11:32
LacP(R0010) + RBS(J61100) + T7ptag(K228000) + terminator(B0015)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K228104_sequence (Version 1) |
Description
T7 polymerase with an amber mutation coding gene is constructed after Lac promoter which would be triggered by IPTG. T7 polymerase can be well expressed in the exsitence of SupD tRNA. Also, the expression of the T7 polymerase can be regulated by RBS. It is one of the LacP-rbs-T7ptag-terminator family members, ditinguished by different rbs.Source
It is made up of standard subparts from the partregistry.| Access | Instance | Definition |
| public public public public public | BBa_R0010 BBa_J61100 BBa_K228000 BBa_B0010 BBa_B0012 | LacI BBa_J61100 BBa_K228000 BBa_B0010 BBa_B0012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0010 BBa_J61100 BBa_K228000 BBa_B0010 BBa_B0012 | 1,200 209,220 227,2899 2908,2987 2996,3036 | LacI BBa_J61100 BBa_K228000 BBa_B0010 BBa_B0012 |