Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuke Wu
Date created: 2009-09-08 11:00:00
Date modified: 2015-05-08 01:11:34
AND GATE lacP+SupD+AraC+RBS(B0032)+T7ptag
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K228824_sequence (Version 1) |
Description
This is a AND GATE in the Ecoli stain JM109.Use IPTG to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
Note: this AND gate make use of the lacI on the F plasmid of JM109, and because of the instability of the F plasmid, the leakage of lacP is significant. For improvement, a lacI generator can be assemble into it.
This gate only works on low copy plasmid, such as pSB4K5, due to the lacI on the F plasmid.
This gate is only one of nine gates(K228823-K228831), which are different in the RBS of T7ptag.
For more information:
refer to K228000(T7ptag) and K228001(SupD)
referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
| Access | Instance | Definition |
| public public public public public public public | BBa_K228822 BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0032 BBa_K228000 BBa_B0015 | BBa_K228822 BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0032 BBa_K228000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K228822 BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0032 BBa_K228000 BBa_B0015 | 1,481 1502,1581 1590,1630 490,1768 1777,1789 1796,4468 4477,4605 | BBa_K228822 BBa_B0010 BBa_B0012 BBa_K228009 BBa_B0032 BBa_K228000 BBa_B0015 |