Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Minerva Trejo, Laura Gomez, Libertad Pantoja, Abraham Avelar, Uriel Urquiza, Fernando Monta??o, Arturo Velarde, Eduardo Soto, Adrian Granados, Willebaldo Garc??a, Enrique Quintana, Carlos Vargas, Enri
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:37
(pT3/T7-GFP-E3-E9-LuxI-T)+(pTet-LuxR-T)+(plux-RFP)+(plux-antisenseT3/T7-T)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K242901_sequence (Version 1) |
Description
This is a device that should confer protection to an E.Coli population against T3 and T7 bacteriophage infection.The system starts detecting RNApol of T3 or T7 by specific promoters. Transcription of toxins and production of AHL starts with the aim of kill the cell before more phages production.
The surrounding cells receive the quorum molecule (AHL), binding with luxR result in activation of plux->transcription of RFP, just as a reporter. Also you start transcription of an antisense against T3 and T7 replication machinery. The last part has the intention to cause a delay in the production cycle of those phages so the device increase its efficiency
Notes
...Source
...| Access | Instance | Definition |
| public public public public public | BBa_K242900 BBa_K094130 BBa_K116640 BBa_I13507 BBa_K242251 | BBa_K242900 BBa_K094130 BBa_K116640 BBa_I13507 BBa_K242251 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K242900 BBa_K094130 BBa_K116640 BBa_I13507 BBa_K242251 | 1,2437 2446,3306 3315,4313 4322,5182 5191,5544 | BBa_K242900 BBa_K094130 BBa_K116640 BBa_I13507 BBa_K242251 |