Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Freiburg Bioware09
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:11:37
His-FluA-Middle Linker-Fok_i
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K243014_sequence (Version 1) |
Description
These part units some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain foka, make it possible to purifier and detect the function of the protein in one step. The fluorescineA tag allows the measurement by quenching and the coupling to a anticalin linked oligo.Notes
The choice of the linker, lipocalin tag and purification tag allows us several different combinations.Source
Combined the parts by serial cloning steps.| Access | Instance | Definition |
| public public public public public public public | BBa_K157011 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K243005 BBa_B0105 BBa_K243001 | His Scar 25 BBa_K157004 Scar 25 MiddleLink Scar 25 Fok_i |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K157011 BBa_B0105 BBa_K157004 BBa_B0105 BBa_K243005 BBa_B0105 BBa_K243001 | 1,18 19,24 25,546 547,552 553,576 577,582 583,1161 | His Scar 25 BBa_K157004 Scar 25 MiddleLink Scar 25 Fok_i |