Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cihan Tastan and Ozkan IS
Date created: 2009-10-09 11:00:00
Date modified: 2015-05-08 01:11:42
Oxygen-dependent EGF synthesizing with GFP reporter
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K258011_sequence (Version 1) |
Description
the Epidermal Growth Factor(EGF) has a big role in wound healing.Moreover, it has also a function to start the formation of keratin and to stimulate cell division. It can also increase the amount of nucleic acids.And, it causes the storage of glikozamin and glikozaminoglikan.In structural perspective, it is composed of 53 aminoacids and has three disulfide bond.Although these positive effect of EGF, with the abundance, it can show ,for example, a trigger effect of tumor cells formation. Another side effect is to start the formation of vein.As a result, it may show a trigger for cancer unless unregulated and too much synthesize..Notes
design with biobrick assemblySource
EGF will be activated with oxygen presence using tetR inverter mechanism. Therefore,with the absence of oxygen tetR will be synthesized so that EGF and GFP synthesize will be stopped.| Access | Instance | Definition |
| public public public public public public public | BBa_K258005 BBa_Q04400 BBa_B0034 BBa_K258010 BBa_K258006 BBa_I13500 BBa_B0015 | BBa_K258005 tetR BBa_B0034 BBa_K258010 BBa_K258006 BBa_I13500 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K258005 BBa_Q04400 BBa_B0034 BBa_K258010 BBa_K258006 BBa_I13500 BBa_B0015 | 1,137 146,1047 1056,1067 1074,1235 1242,2669 2678,3415 3424,3552 | BBa_K258005 tetR BBa_B0034 BBa_K258010 BBa_K258006 BBa_I13500 BBa_B0015 |