Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Siming Ma
Date created: 2009-09-17 11:00:00
Date modified: 2015-05-08 01:11:45
CrtEBI under constitutive promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K274110_sequence (Version 1) |
Description
This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011.Enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274110 converts colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene).
Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
Notes
The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate).Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
Source
Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.Please refer to Part BBa_K274100 for more information on individual components.
| Access | Instance | Definition |
| public public public public public public public | BBa_R0011 BBa_K118012 BBa_I742151 BBa_J15001 BBa_K118002 BBa_J15001 BBa_K118003 | lacI+pL BBa_K118012 BBa_I742151 BBa_J15001 BBa_K118002 BBa_J15001 BBa_K118003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_K118012 BBa_I742151 BBa_J15001 BBa_K118002 BBa_J15001 BBa_K118003 | 1,54 64,67 74,985 994,1003 1010,1942 1951,1960 1967,3448 | lacI+pL BBa_K118012 BBa_I742151 BBa_J15001 BBa_K118002 BBa_J15001 BBa_K118003 |