Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Siming Ma
Date created: 2009-09-18 11:00:00
Date modified: 2015-05-08 01:11:45
CrtEBIY under pBad promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K274220_sequence (Version 1) |
Description
This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under arabinose-induced promoter I0500.Enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 converts colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene).
Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone).
Notes
The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate).Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
Source
Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.Please refer to Part BBa_K274200 for more information on individual components.
| Access | Instance | Definition |
| public public public public public public public public public | BBa_I0500 BBa_K118012 BBa_I742151 BBa_J15001 BBa_K118002 BBa_J15001 BBa_K118003 BBa_K118012 BBa_K118008 | pBad/araC BBa_K118012 BBa_I742151 BBa_J15001 BBa_K118002 BBa_J15001 BBa_K118003 BBa_K118012 BBa_K118008 |