Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:47
3L.3_23.A1
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K277134_sequence (Version 1) |
Description
3L.3_23.A1 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns,repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1 is comprised of and must be constructed from the parts 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.This part wholly contains the following features (positions offset from first base of sequence):
kind and name offset notes
loxP_site loxpsym_delyeast103_25(chrIII)3000..3001 (3000..3033)
gene YCL073C (413..2260) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W
loxP_site loxPsym_YCL063W (8632..8665)
gene YCL069W (3672..5048) Permease of basic amino acids in the vacuolar membrane
ARS ARS320 (5534..6341) Autonomously Replicating Sequence on Chromosome III
loxP_site loxpsym_delyeast_chr3_3_17(chrIII)5500..9500 (5500..5533)
reverse_primer YCL063W_tagr1v1 (8223..8250)
loxP_site loxpsym_delyeast103_24(chrIII)1..6100 (1..34)
mutation_affecting_coding_sequence YCL069W_re_remove_AlwNI (4680..4691) removal of AlwNI
forward_primer YCL064C_tagf1v1 (5973..6000)
loxP_site loxPsym_YCL069W (5052..5085)
gene YCL063W (7357..8628) Protein involved in vacuole inheritance%3B acts as a vacuole-specific receptor for myosin Myo2p
forward_primer YCL063W_tagf1v1 (7971..7998)
reverse_primer YCL064C_tagr1v1 (6216..6243)
ARS ARS301 (5146..5401) Inactive replication origin associated with the silent mating type locus HML%2C where it functions as a transcriptional silencer
gene YCL064C (5865..6947) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine
Sequence (corresponding to coordinates 1..8879 in synthetic chromosome yeast_chr3_3_23)
Notes
Constructed using USER assembly method described in RFC38Source
Synthetic Yeast| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_K277001 BBa_K277002 BBa_K277003 BBa_K277004 BBa_K277005 BBa_K277006 BBa_K277007 BBa_K277008 BBa_K277009 BBa_K277010 BBa_K277011 BBa_K277012 | BBa_K277001 BBa_K277002 BBa_K277003 BBa_K277004 BBa_K277005 BBa_K277006 BBa_K277007 BBa_K277008 BBa_K277009 BBa_K277010 BBa_K277011 BBa_K277012 |