Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James DiCarlo
Date created: 2009-10-20 11:00:00
Date modified: 2015-05-08 01:11:47
3L.3_23.B1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K277136_sequence (Version 1) |
Description
3L.3_23.B1 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns,repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1 is comprised of and must be constructed from the parts 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.This part wholly contains the following features (positions offset from first base of sequence):
kind and name offset notes
reverse_primer YCL054W_tagr3v1 (4762..4789)
gene YCL050C (8947..9912) Diadenosine 5'%2C5-P1%2CP4-tetraphosphate phosphorylase I (AP4A phosphorylase)%2C involved in catabolism of bis(5'-nucleosidyl) tetraphosphates%3B has similarity to Apa2p
mutation_affecting_coding_sequence YCL054W_re_remove_Bpu10I (4706..4714) removal of Bpu10I
loxP_site loxPsym_YCL051W (8663..8696)
reverse_primer YCL054W_tagr1v1 (4321..4348)
forward_primer YCL051W_tagf1v1 (8263..8290)
reverse_primer YCL050C_tagr1v1 (9442..9469)
mutation_affecting_coding_sequence YCL054W_re_remove_MmeI (3008..3019) removal of MmeI
mutation_affecting_coding_sequence YCL054W_re_remove_BlpI (3947..3955) removal of BlpI
mutation_affecting_coding_sequence YCL051W_re_remove_MmeI (7637..7648) removal of MmeI
ARS ARS304 (1243..1700) Autonomously Replicating Sequence on Chromosome III
gene YCL051W (6908..8659) Protein involved in control of cell wall structure and stress response%3B inhibits Cbk1p protein kinase activity%3B overproduction confers resistance to cell-wall degrading enzymes
reverse_primer YCL054W_tagr2v1 (3100..3127)
reverse_primer YCL052C_tagr1v1 (6161..6188)
forward_primer YCL051W_tagf2v1 (7321..7348)
forward_primer YCL052C_tagf1v1 (5771..5798)
mutation_affecting_coding_sequence YCL050C_re_remove_MmeI (9502..9513) removal of MmeI
forward_primer YCL054W_tagf2v1 (2680..2707)
forward_primer YCL054W_tagf1v1 (3916..3943)
forward_primer YCL054W_tagf3v1 (4486..4513)
forward_primer YCL050C_tagf1v1 (9115..9142)
reverse_primer YCL051W_tagr2v1 (7789..7816)
gene YCL052C (5186..6436) Essential component of glycosylphosphatidylinositol-mannosyltransferase I%2C required for the autocatalytic post-translational processing of the protease B precursor Prb1p%2C localizes to ER in lumenal orientation%3B homolog of mammalian PIG-X
reverse_primer YCL051W_tagr1v1 (8518..8545)
loxP_site loxPsym_YCL050C (8910..8943)
gene YCL054W (2492..5017) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants
Sequence (corresponding to coordinates 19161..29646 in synthetic chromosome yeast_chr3_3_23)
Notes
Constructed using USER assembly method described in RFC38Source
Synthetic Yeast| Access | Instance | Definition |
| public public public public public public public public public public public public public public | BBa_K277027 BBa_K277028 BBa_K277029 BBa_K277030 BBa_K277031 BBa_K277032 BBa_K277033 BBa_K277034 BBa_K277035 BBa_K277036 BBa_K277037 BBa_K277038 BBa_K277039 BBa_K277040 | BBa_K277027 BBa_K277028 BBa_K277029 BBa_K277030 BBa_K277031 BBa_K277032 BBa_K277033 BBa_K277034 BBa_K277035 BBa_K277036 BBa_K277037 BBa_K277038 BBa_K277039 BBa_K277040 |