Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Charoy
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:49
Double terminator + Kanamycin resistance cassette + Double ter
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K292004_sequence (Version 1) |
Description
This part contains a double terminator and a functional kanamycin resistance cassette (promoter, resistance against kanamycin and a double terminator, BBa_B0014 + BBa_P1003 + BBa_B0014). It is reliable to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage.In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin.
We can use this part as a reporter, or as a simple resistance cassette.
The double terminator allows the RNA translation arrest before the resistance cassette, so this is already created to insert a coding gene with its RBS before the resistance cassette.
Notes
When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.Source
Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23)