Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Charoy
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:49
Strong RBS + LacI repressor + double terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K292006_sequence (Version 1) |
Description
This part contains a strong ribosome binding site, the LacI repressor and a double terminator (BBa_B0030 + BBa_C0012 + BBa_B0014). This brick comes from the BBa_K292005. The LacI repressor represses pLac promoter from the E.coli lactose operon. So LacI repressor and this bio-brick are really reliable to use when we want to control a gene expression by using pLac promoter. In fact when LacI is not expressed, pLac is activated, and when there is an expression of LacI, pLac is inhibited. We can easily design a system to induce the transcription of LacI by using another operon as the tetracycline operon and creates a failed feedback mechanism for pLac inhibition and so, for gene expression inhibition.The LacI (BBa_K292006) already contains a strong RBS and a double terminator, so this brick is ready to use!
History:
In E.Coli, pLac is a part of the lactose operon. LacI is constantly expressed and represses the pLac. In the presence of Lactose, LacI is inhibited and pLac is active and allows the transcription of beta-galactosidase. Lactose can be substituted by IPTG (Isopropyl β-D-1-thiogalactopyranoside).
Notes
N/ASource
Part:BBa_B0030, Designed by Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. Group: NCBS, Prather LabPart:BBa_C0012, Designed by Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton Group: Registry
Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)