Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Philip Hall
Date created: 2010-10-24 11:00:00
Date modified: 2015-05-08 01:11:52
Sucrose-limitation induced kill switch
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K302035_sequence (Version 1) |
Description
Encodes a stable non-specific ribonuclease toxin (mazF) and its inhibitory antitoxin (mazE) in Bacillus Subtilis. Contains Sucrose sensitive inducer that will only allow coding sequence translation in the presence of sucrose. These genes are used in Bacillus Subtilis to provide a toxin-antitoxin kill switch in various stressful conditions. When translation of both genes is turned off by sucrose limitation mazE will be degraded faster than mazF. There is then no inhibiton of mazF, killing the cell.Notes
Sequences selected based upon the work of Shimotsu, H., and Henner, D. J. (1986) J. Bacteriol. 168, 380???388 and Pellegrini, O., Mathy, N., Gogos, A., Shapiro, L., and Condon, C. (2005) The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor. Mol Microbiol 56: 1139???1148.Source
Bacillus Subtilis 168 strain| Access | Instance | Definition |
| public public public public | BBa_K143012 BBa_K302031 BBa_K302034 BBa_B0014 | Pveg BBa_K302031 BBa_K302034 BBa_B0014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K143012 BBa_K302031 BBa_K302034 BBa_B0014 | 1,97 106,288 297,903 912,1006 | Pveg BBa_K302031 BBa_K302034 BBa_B0014 |