Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: IC 2010 Team
Date created: 2010-10-19 11:00:00
Date modified: 2015-05-08 01:11:57
Pveg-spoVG-LytC-Glycine Linker-TEV Cleavage Site-AIP-His Tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K316032_sequence (Version 1) |
Description
We designed a protein that carries our signal peptide ComC out of the cell where a protease has access to it. The protease can then proceed to cleave ComC off the protein, allowing quorum sensing via the the ComCDE system to take place. One big problem we have to overcome is the cell wall that will obstruct the protease???s access to the protein carrying the signal peptide. The Detection module consists of a cell wall anchor, a signal peptide called ComC, and a linker that connects the two and is specifically designed to be cleaved by the protease we want to detectNotes
Pfu DNA polymerase was used to minimise PCR errorsSource
Genome PCR from B. subtilis genome (strain) with linker-TEV cleavable site-ComC synthesised and attached via a PME site within LytC gene| Access | Instance | Definition |
| public public public | BBa_K316001 BBa_K316042 BBa_B0014 | Pveg LytC-Gly-T BBa_B0014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K316001 BBa_K316042 BBa_B0014 | 1,97 106,1245 1254,1348 | Pveg LytC-Gly-T BBa_B0014 |