| Types | DnaRegion
|
| Roles | engineered_region
Generator
|
| Sequences | BBa_K318011_sequence (Version 1)
|
Description
This composite part is an inducible Hin DNA recombinase generator which acts on the hixC recombination sites. It is composed of the following biobricks: BBa_B1006, BBa_K200021, BBa_J31000, BBa_B1006.
Notes
To minimize potential transcription due to upstream promoter activity, a terminator was placed upstream of the pLac promoter. A terminator was placed downstream of the Hin encoding sequence to end transcription after the recombinase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.
Source
Biobricks
