Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: William Rostain
Date created: 2010-10-07 11:00:00
Date modified: 2015-05-08 01:11:59
Lac promoter + 356K firefly luciferase mutant (red colour)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K322212_sequence (Version 1) |
Description
This part is made of the lac promoter followed by 356K firefly luciferase mutant, which shifts its emission spectrum to make it red. Note that firefly luciferase is temperature sensitive, and cells grown at 30??C are considerably brighter than cells grown at 37??C.The luciferase uses luciferin as a substrate, which needs to be added to the medium.
Notes
Note that the luciferase is temperature sensitive and cells grown at 30??C are a lot brighter that cells grown at 37??C.Luciferin penetrates the cell much better at low pH. If the cells are spun down, then resuspended in citrate buffer, pH4.8, they are much brighter.
Source
The source is BBa_I712019, which comes from the promega PGL3 vector.| Access | Instance | Definition |
| public public public | BBa_J33207 BBa_J15001 BBa_K322211 | BBa_J33207 BBa_J15001 BBa_K322211 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_J15001 BBa_K322211 | 1,600 609,618 625,2280 | BBa_J33207 BBa_J15001 BBa_K322211 |