BBa_K325219

BBa_K325219 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K325219
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bill Collins and Emily Knott
Date created: 2010-09-14 11:00:00
Date modified: 2015-05-08 01:12:02

Red Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)



    • Details

    Types
    DnaRegion

    Roles
    Generator

    engineered_region

    Sequences BBa_K325219_sequence (Version 1)

    Description

    This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata). It also generates the luciferin regenerating enzyme. When luciferin and D-cysteine are added this BioBrick should give sustained light output. With simply luciferin it will give a burst of light output.

    Notes

    Biobrick assembly

    Source

    Biobrick assembly from its two subparts

    Access Instance Definition
    public
    public
    		BBa_I0500
    BBa_K325210
    		pBad/araC
    BBa_K325210

    Sequence Annotation Location Component / Role(s)
    BBa_I0500
    BBa_K325210
    		1,1210
    1219,3841
    		pBad/araC
    BBa_K325210

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K325219/1
     

    Attachments

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