Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: KUO, CHIA-CHUN
Date created: 2010-10-21 11:00:00
Date modified: 2015-05-08 01:12:05
Constitutive promoter+37℃
induced RBS+tetR+double terminator+Ptet+RBS+GFP+double terminator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K332032_sequence (Version 1) |
Description
We want to utilize a temperature-regulated mechanism to control the expression period of a crystal protein. Therefore, we took advantage of the interaction between 37?? C induced RBS, tetR gene and pTet promoter to create the temperature regulating circuit. The following is a description of our prescribed strategy.First, we use the constitutive promoter, 37?? C induced RBS (K115002), the tetR gene and terminators. We will call this part the ???Regulator???.
Notes
noneSource
none| Access | Instance | Definition |
| public public public public public | BBa_K332031 BBa_R0040 BBa_B0034 BBa_E0040 BBa_J61048 | BBa_K332031 p(tetR) BBa_B0034 GFP BBa_J61048 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K332031 BBa_R0040 BBa_B0034 BBa_E0040 BBa_J61048 | 1,907 916,969 978,989 996,1715 1724,1836 | BBa_K332031 p(tetR) BBa_B0034 GFP BBa_J61048 |