B-carotene

BBa_K343006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K343006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Christian Kurtzhals
Date created: 2010-10-13 11:00:00
Date modified: 2015-05-08 01:12:08

Expresses B-carotene monooxygenase on a constitutive promotor



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Generator

    Sequences BBa_K343006_sequence (Version 1)

    Description

    This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction:

    beta-carotene + O2 = 2 retinal

    Notes

    The brick requires B-carotene supply to function. This can either be supplied externally, or alternatively the BBa_K274210 B-carotene generator will induce full B-carotene synthesis in E. coli.

    Source

    The ninaB gene was originally cloned from drosophila melanogaster, and cDNA was aquired for production of the biobrick from the Drosophila Genome Ressource Center. Promotor+rbs brick BBa_J13002 and terminator BBa_B0015 were used.

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K343006/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)