Types | DnaRegion
|
Roles | Signalling
engineered_region
|
Sequences | BBa_K359000_sequence (Version 1)
|
Description
S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism including the AIP sensor and generator of AIP (auto-inducer peptide).
Notes
Used J23017, a mild constitutive promoter to avoid inserting too many unnatural proteins into the E.coli membrane.
Source
These parts were designed and constructed by the Cambridge 2007 iGEM Team:
The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.