BBa_K359000

BBa_K359000 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K359000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leah Kocsis
Date created: 2010-08-17 11:00:00
Date modified: 2015-05-08 01:12:12

AIP sensor and generator



Types
DnaRegion

Roles
Signalling

engineered_region

Sequences BBa_K359000_sequence (Version 1)

Description

S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism including the AIP sensor and generator of AIP (auto-inducer peptide).

Notes

Used J23017, a mild constitutive promoter to avoid inserting too many unnatural proteins into the E.coli membrane.

Source

These parts were designed and constructed by the Cambridge 2007 iGEM Team:
The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K359000/1