Types | DnaRegion
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Roles | engineered_region
Intermediate
|
Sequences | BBa_K359006_sequence (Version 1)
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Description
S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism including the AIP sensor and generator of AIP (auto-inducer peptide).
Notes
Used J23107, a mild constitutive promoter, to avoid inserting too many unnatural proteins into the E.coli membrane.
Source
These parts were designed and constructed by the Cambridge 2007 iGEM Team: The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.