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BBa_K389403

BBa_K389403 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K389403

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jonas Aretz

Date created: 2010-10-10 11:00:00

Date modified: 2015-05-08 01:12:19

Sensitivity tuner + luciferase

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_K389403_sequence (Version 1)

Description

This is a modified BBa_I746390 sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.

Notes

arabinose promoter removed, GFP exchanged with luciferase compared to the original part BBa_I746370

Source

luciferase from pGL4.10luc2 (Promega)
the rest from the partsregistry

Components

Access	Instance	Definition
public public public public public public public	BBa_I746352 BBa_B0034 BBa_E1010 BBa_B0015 BBa_I746360 BBa_B0034 BBa_K389004	BBa_I746352 BBa_B0034 mRFP1 BBa_B0015 BBa_I746360 BBa_B0034 luc

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I746352 BBa_B0034 BBa_E1010 BBa_B0015 BBa_I746360 BBa_B0034 BBa_K389004	1,264 273,284 291,996 1005,1133 1142,1232 1241,1252 1259,2914	BBa_I746352 BBa_B0034 mRFP1 BBa_B0015 BBa_I746360 BBa_B0034 luc

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K389403/1