BBa_K398305

BBa_K398305 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K398305

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eva Brinkman

Date created: 2010-10-09 11:00:00

Date modified: 2015-05-08 01:12:22

AlkS_E0240

Details

• Types: DnaRegion
• Roles: Intermediate; engineered_region
• Sequences: BBa_K398305_sequence (Version 1)

Description

AlkS transcriptional regulator activates the expression of its own gene, and that of alkT, from a promoter named PalkS2 in the presence of alkanes. . AlkS levels that are high enough to activate the expression of the alkBFGHJKL operon from the PalkB promoter. AlkS recognizes C5???C10 n-alkanes as effectors, but does not respond to shorter or larger alkanes.

Notes

Nucleotide sequence optimized for expression in E.coli

Source

Genbank

Components

Access	Instance	Definition
public	BBa_K398300	BBa_K398300
public	BBa_B0032	BBa_B0032
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K398300	1,2649	BBa_K398300
BBa_B0032	2658,2670	BBa_B0032
BBa_E0040	2677,3396	GFP
BBa_B0010	3405,3484	BBa_B0010
BBa_B0012	3493,3533	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K398305/1

Member of these Collections

• iGEM Parts Registry