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BBa_K429001

BBa_K429001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K429001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stefano Fenu

Date created: 2010-10-25 11:00:00

Date modified: 2015-05-08 01:12:29

Promoter Testing Construct

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K429001_sequence (Version 1)

Description

This part is designed to act as a testing chasis for new promoters. A promoter can be extended from the B0034 end of the part by PCR extension, and if promoter activity is repressed GFP will be expressed.

Notes

The sequence was designed to be an alternative to K429000 with stronger expression of GFP.

Source

The part is a composite of BBa_Q04510 and BBa_I13504

Components

Access	Instance	Definition
public public public public public public public public public	BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012 BBa_R0051 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	BBa_B0034 cI lam BBa_B0010 BBa_B0012 cI lam BBa_B0034 GFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034 BBa_C0051 BBa_B0010 BBa_B0012 BBa_R0051 BBa_B0034 BBa_E0040 BBa_B0010 BBa_B0012	1,12 19,793 802,881 890,930 939,987 996,1007 1014,1733 1742,1821 1830,1870	BBa_B0034 cI lam BBa_B0010 BBa_B0012 cI lam BBa_B0034 GFP BBa_B0010 BBa_B0012