Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31
Repressible Tango Ligand Generator (Hef1a-LacO-pDisplay-Vasopressin-MYC) MammoBlock Device
| Types | DnaRegion |
| Roles | engineered_region Signalling |
| Sequences | BBa_K511809_sequence (Version 1) |
Description
This MammoBlock composite device produces the pDisplay-Vasopressin-MYC synthetic ligand designed to activate expression from the AVPR2-TEVs-Gal4-VP16 signaling receptor. Because this ligand is displayed in a membrane-bound, extracellular fashion, it is ideal for juxtacrine signaling that is orthogonal to other juxtacrine signaling systems, particularly the Notch-Delta system. Transcription is driven by the Hef1a-LacO promoter and therefore repressible by LacI variants.Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accomodations to provide our complete expression vectors.Source
Consult constituent parts.| Access | Instance | Definition |
| public public public | BBa_K511000 BBa_J85600 BBa_K511500 | BBa_K511000 BBa_J85600 BBa_K511500 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K511000 BBa_J85600 BBa_K511500 | 1,1275 1276,1297 1298,1570 | BBa_K511000 BBa_J85600 BBa_K511500 |