BBa_K511814

BBa_K511814 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K511814
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31

Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device



Types
DnaRegion

Roles
Signalling

engineered_region

Sequences BBa_K511814_sequence (Version 1)

Description

This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces Delta-mCherry at a low, basal (OFF) level.

Notes

In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.

Source

Consult constituent components.

igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K511814/1