Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grant Robinson
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:31
Inducible Fluorescence/Repression Generator (UAS-Gal4-amCyan-miRFF4) MammoBlock Device
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K511821_sequence (Version 1) |
Description
This MammoBlock composite device produces the cyan fluorescent protein amCyan-miRFF4 when induced with Gal4 transactivator variants and otherwise produces amCyan-miRFF4 at a low, basal (OFF) level. Note that the presence of the miRFF4 micro RNA tag on amCyan means this MammoBlock device also acts as a repressor.Notes
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors.Source
Consult constituent components.| Access | Instance | Definition |
| public public public | BBa_K511003 BBa_J85600 BBa_K511103 | BBa_K511003 BBa_J85600 BBa_K511103 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K511003 BBa_J85600 BBa_K511103 | 1,220 221,242 243,1308 | BBa_K511003 BBa_J85600 BBa_K511103 |