Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Masato Ohgishi
Date created: 2011-10-02 11:00:00
Date modified: 2015-05-08 01:12:33
sulA promoter evaluation device
| Types | DnaRegion |
| Roles | Measurement engineered_region |
| Sequences | BBa_K518013_sequence (Version 1) |
Description
Microbes including Escherichia coli are known to respond to various DNA-injuring stress (ionizing radiation, ultraviolet radiation, peroxides etc...), altering their gene expressions. This response, known as "SOS response", are induced by regulatory protein called RecA, which is activated when binds to single-strand DNA. DNA-RecA complex promotes the self-degradation of LexA, a common repressor for SOS genes.SulA is responsible for stress-induced halt of cell division. The promoter of sulA, or sulAp, is acutely induced by various stress factors, including ultraviolet irradiation.
We provide a sulAp evaluation device to make it easy to measure the varying expression of sulAp in various "stressful" conditions. Employing a dual luciferase reporter construct, quantitative measurement and comparison of Relative Promoter Unit (RPU) is achieved.
Notes
sulAp + dual luciferase reporter constructSource
sulAp (BBa_K518010) was cloned from Escherichia coli K12 strain's genome.| Access | Instance | Definition |
| public public | BBa_K518010 BBa_K518002 | sulAp BBa_K518002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K518010 BBa_K518002 | 1,52 61,2944 | sulAp BBa_K518002 |