BBa_K542006

BBa_K542006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K542006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Vuong
Date created: 2011-09-22 11:00:00
Date modified: 2015-05-08 01:12:39

pBAD Inverse-Regulated Arg-tagged ECFP and EYFP (FRET Reporter)



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K542006_sequence (Version 1)

Description

Made by assembling BBa_K542003 with BBa_K542005.

The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP and Arg-tagged EYFP will be produced constitutively. http://partsregistry.org/wiki/index.php?title=Part:BBa_K542003 BBa_K542003] was placed upstream of BBa_K542005, so that expression of the fluorescence proteins maybe "turned off" in the presence of arabinose.

Fluorescence/F??rster Resonance Energy Transfer(FRET) is a distance-dependent phenomenon in which the excitation of a donor fluorophore leads to emission by an acceptor fluorophore; this occurs if the two fluorophores are within a certain distance to each other. The distance is dependent on the FRET pair used. The emission spectrum of the donor fluorophore must overlap with the excitation spectrum of the acceptor fluorophore for FRET to occur. FRET is explained in further detail on the Lethbridge 2009 Wiki. This phenomenon will allow for characterizing co-localization within the Lumazine Synthase microcompartment.

Notes

The FRET portion to the "Co-localization Construct".

Source

BBa_K542003 and BBa_K542005 were both assembled by the Letbridge 2011 team.

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BBa_K542006/1