Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhang Chao
Date created: 2011-09-24 11:00:00
Date modified: 2015-05-08 01:12:41
Blue Light Sensor+PtrpL+mRFP1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K560000_sequence (Version 1) |
Description
LovTAP Photosensor is a chimeric protein, which consists of LOV domain which senses blue light. It fused to the trpR-DNA binding domain of the transcription factor trp repressor.Under the presence of blue light, maximum at 450nm, the absorption of a photon leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.
Notes
This system had better work in TrpR mutant E.coli strain.Source
LovTAP was assembled by Strickland et al.| Access | Instance | Definition |
| public public public public public | BBa_J23117 BBa_K360121 BBa_K360023 BBa_B0034 BBa_E1010 | BBa_J23117 BBa_K360121 BBa_K360023 BBa_B0034 mRFP1 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23117 BBa_K360121 BBa_K360023 BBa_B0034 BBa_E1010 | 1,35 44,887 896,944 953,964 971,1676 | BBa_J23117 BBa_K360121 BBa_K360023 BBa_B0034 mRFP1 |