| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_K577895_sequence (Version 1)
|
Description
A composite part containing the TetR gene, C0040, turned on by a constitutive promoter, I14033. The TetR represses the pTet promoter in front of the RFP gene. By addition of tetracycline or aTc, this repression is inhibited and the cells should fluoresce red.
Notes
The whole part is in the backbone of I14033. P0440, the TetR composite part was used and inserted into its respective promoter. The RBS+RFP+Terminator part came from the backbone J61102 and was inserted into the pTet promoter. The pTet+RFP part was then combined as a suffix insertion to the I14033+TetR backbone.
Source
Spring 2011 iGEM Kit Plates
