Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lei Sun
Date created: 2011-09-20 11:00:00
Date modified: 2015-05-08 01:12:49
PompC-tetR inverter-B0034-mCherry
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K592021_sequence (Version 1) |
Description
Red light output system that produces mCherry. PompC is regulated by Cph8/EnvZ red light-sensing system. Due to the nature of how Cph8 works, the light sensor is shut off by red light and activated in darkness. In order to construct a light sensing system that gives positive output by illumination and no output when in dark, using an inverter becomes necessary.Notes
The reason tetR inverter was picked over lambda C1 is because lambda C1 is already used in BBa_K592020. Therefore, if these two systems are to work in the same vector, one has to use an alternative inverter.Source
All components used came from the iGEM 2011 DNA distribution.| Access | Instance | Definition |
| public public public public | BBa_R0082 BBa_Q04400 BBa_B0034 BBa_J06505 | OmpR tetR BBa_B0034 mCherry |