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BBa_K604007

BBa_K604007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K604007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ruojun wang

Date created: 2011-10-04 11:00:00

Date modified: 2015-05-08 01:12:51

autoinducer synthetase rhlI and GFP under PyeaR

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K604007_sequence (Version 1)

Description

The pathway detects nitrates in the medium; if KNO3 is present, the circuit is activated, RhlI produces the AHL (C4-homoserine lactone) and TetR is synthesized to block another pathway. GFP is emitted to show the start of transcription.

Notes

use low copy number plasmid

Source

combination of several existing parts in the registry

Components

Access	Instance	Definition
public public public public public public public	BBa_K216005 BBa_B0034 BBa_C0070 BBa_B0034 BBa_C0041 BBa_B0034 BBa_E0040	BBa_K216005 BBa_B0034 rhII BBa_B0034 tetR BBa_B0034 GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K216005 BBa_B0034 BBa_C0070 BBa_B0034 BBa_C0041 BBa_B0034 BBa_E0040	1,100 109,120 127,793 802,813 820,1445 1454,1465 1472,2191	BBa_K216005 BBa_B0034 rhII BBa_B0034 tetR BBa_B0034 GFP

Other Properties

igem#experience	Works
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K604007/1