Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Camille de Froberville
Date created: 2011-10-22 11:00:00
Date modified: 2015-05-08 01:12:51
T7 amber expression monitored by a linear system
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K606063_sequence (Version 1) |
Description
This system consists in the association of a pT7 GFP reporter with the constitutive expression of a T7 amber.This design aims at quantifying T7 amber expression with the GFP fluorescence
Notes
To design the T7 amber we had to consider how many amber sites we would put there for it to be the less leaky possible.Source
The T7 amber polymerase is from the classic T7 polymerase (part BBa_K145001).| Access | Instance | Definition |
| public public public public | BBa_B0010 BBa_B0012 BBa_K606035 BBa_K606033 | BBa_B0010 BBa_B0012 pT7 GFP T7 BBa_K606033 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0010 BBa_B0012 BBa_K606035 BBa_K606033 | 778,857 866,906 1,1067 1076,3990 | BBa_B0010 BBa_B0012 pT7 GFP T7 BBa_K606033 |