Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: K??vin Yauy
Date created: 2011-10-22 11:00:00
Date modified: 2015-05-08 01:12:51
T7 amber with autoamplification loop
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K606064_sequence (Version 1) |
Description
This design are the receiver system for our tRNA diffusion system. If the bacteria receives tRNA Amber, their will be an expression of T7 Amber and autoamplification of T7 monitored by GFP.Notes
To design the T7 amber we had to consider how many amber sites we would put there for it to be the less leaky possible. Our choice is 2 mutations Amber.Source
The T7 amber polymerase is from the classic T7 polymerase (part BBa_K145001).| Access | Instance | Definition |
| public public public public | BBa_B0010 BBa_B0012 BBa_K606036 BBa_K606033 | BBa_B0010 BBa_B0012 T7 auto-am BBa_K606033 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0010 BBa_B0012 BBa_K606036 BBa_K606033 | 3459,3538 3547,3587 1,3748 3757,6671 | BBa_B0010 BBa_B0012 T7 auto-am BBa_K606033 |