LacI-pHS-t

BBa_K606066 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K606066

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: K??vin Yauy

Date created: 2011-10-22 11:00:00

Date modified: 2015-05-08 01:12:51

Upstream LacI expression IPTG inducible tRNA emitter

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K606066_sequence (Version 1)

Description

This device are the emitter system for our tRNA diffusion system. It express the tRNA amber who is needed for expression of T7 Amber. In addition a strong LacI expression cassette is added to repress pHyperspank because B. subtilis doesn't express LacI constitutively.

Notes

This sequence is the pure tRNA without the side sequences. This part was initially designed for B.subtillis but it also works in E.coli (look at our experience page BBa_K606034).

Source

Synthetize de novo by matching primers and cloned from the tRNA cluster of B. Subtilis genome of strain 168

Components

Access	Instance	Definition
public	BBa_K606010	BBa_K606010
public	BBa_K606041	pHS-tRNAAm

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K606010	1,1346	BBa_K606010
BBa_K606041	1355,1676	pHS-tRNAAm

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K606066/1

Member of these Collections

• iGEM Parts Registry