BBa_K639000

BBa_K639000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K639000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eirik Selsaas
Date created: 2011-08-25 11:00:00
Date modified: 2015-05-08 01:12:57

LacI-pLac-mCherry with RBS and terminator



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K639000_sequence (Version 1)

Description

This brick will yield red colonies as it is. When a promoter is inserted in front of the brick, the red color will disappear or be weaker, due to production of LacI that repress lac-promoter. One way of using this brick is to insert a promoter that you suspect, or know is repressed by a molecule or other stimulus. When the promoter is inhibited, transcription of LacI will stop and mCherry will be produced. Thus, the stimuli can be directly measured as intensity of red colour or fluorescense.

Notes

None

Source

This is a composite part and is made only from parts in the registry.

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K639000/1