Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jim Rose
Date created: 2011-07-04 11:00:00
Date modified: 2015-05-08 01:12:59
Fast-Fusion GFP-Xyle Reporter (RecA cleaveage with long linker)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K648022_sequence (Version 1) |
Description
This is a fast-acting reporter using the Xyle gene (BBa_K648011) fused to GFP. In its normal un-cleaved state the polymerization ability necessary for enzymatic action is inactivated. Once the fusion protein is cleaved by the RecA, which is activated by damaged DNA, the catechol-2,3-dioxygenase enzyme encoded by the Xyle gene is able to convert catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. This reporter is able to produce a visible color response in a matter of minutes.This version uses the RecA cleavage site (BBa_K648009) followed by the longest of the three flexible fusion protein linkers (BBa_K648006). The linker is attached to the N-terminus of the Xyle gene.
Notes
All parts were assembled via standard 25 assembly methods.Source
The design of this part was inspired by the Imperial College London 2010 iGEM Team's Fast-Response module, part BBa_K316007.| Access | Instance | Definition |
| public public public public public public public | BBa_J23100 BBa_B0034 BBa_K648013 BBa_K648009 BBa_K648006 BBa_K648011 BBa_B0015 | BBa_J23100 BBa_B0034 BBa_K648013 BBa_K648009 BBa_K648006 BBa_K648011 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K648013 BBa_K648009 BBa_K648006 BBa_K648011 BBa_B0015 | 1,35 44,55 64,798 807,827 836,865 874,1791 1800,1928 | BBa_J23100 BBa_B0034 BBa_K648013 BBa_K648009 BBa_K648006 BBa_K648011 BBa_B0015 |