Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yidan Hu
Date created: 2011-09-19 11:00:00
Date modified: 2015-07-08 03:16:04
a bacteria population-control device with RBS1.0 driven by lacl+pL
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K658001_sequence (Version 1) |
Description
The device is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. The LuxI protein synthesizes a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of the killer gene ccdB under the control of a promoter lux pR.Sufficiently high levels of CcdB which is a bacterial toxin that targets DNA gyrase cause cell death.Low cell density doesn???t have the ability to produce sufficient LuxR/AHL complex to activate the promoter lux pR. The programmed death circuit ends and the cell density increases. When the cells reach a certain concentration, the death circuit is restarted. Back and forth, the programmed death is achieved in the dynamic process of growth and death. In that way, the bacteria population is programmed to maintain one certain cell density.
Notes
The bacteria population-control system was once a two-plasmid system. In order to enhance its stability and efficiency, we integrated target sequences into one plasmid.Source
K176003| Access | Instance | Definition |
| public public public public public public | BBa_R0011 BBa_F1610 BBa_K658000 BBa_B0034 BBa_K176003 BBa_B0015 | lacI+pL BBa_F1610 BBa_K658000 BBa_B0034 lacZa-ccdB BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_F1610 BBa_K658000 BBa_B0034 BBa_K176003 BBa_B0015 | 1,54 64,861 870,1931 1940,1951 1958,2437 2446,2574 | lacI+pL BBa_F1610 BBa_K658000 BBa_B0034 lacZa-ccdB BBa_B0015 |