Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yidan Hu
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:13:02
a bacteria population-control device with RBS0.07 driven by lacl+pL
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K658005_sequence (Version 1) |
Description
The device is a twin part of Biobrick part BBa_K658001 and BBa_K658003. This device is made up of three subparts: a LuxI produce, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has designed a series of bacteria population-control devices using RBSs of different strength in the killer protein producer. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable stage. RBS0.07 was used to regulate the expression of killer protein ccdB.Notes
Design considerations are the same as BBa_K658001Source
A twin part of BBa_K658001 and BBa_K658003| Access | Instance | Definition |
| public public public public public public | BBa_R0011 BBa_F1610 BBa_K658000 BBa_B0031 BBa_K176003 BBa_B0015 | lacI+pL BBa_F1610 BBa_K658000 BBa_B0031 lacZa-ccdB BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_F1610 BBa_K658000 BBa_B0031 BBa_K176003 BBa_B0015 | 1,54 64,861 870,1931 1940,1953 1960,2439 2448,2576 | lacI+pL BBa_F1610 BBa_K658000 BBa_B0031 lacZa-ccdB BBa_B0015 |